名称：原装正品| rTIMP-1 DuoSet
TECHNICAL HINTS AND LIMITATIONS
A basic understanding of immunoassay development is
required for the successful use of these reagents in immunoassays. Remove any remaining Wash Buffer by inverting the plate and blotting it against clean paper towels. This procedure will produce an adequate but less precise fit of the data. The following factors at 50 ng/mL were assayed and exhibited no cross-reactivity or interference.
Use a fresh reagent reservoir and pipette tips for each step. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. This may interfere with the sensitivity of the assay. Buffers containing a large quantity of protein should be made under sterile conditions and stored at 2 - 8° C or be prepared fresh daily.
The Stop Solution suggested for use with this kit is an acid solution. The diluent suggested in this protocol should be suitable for most cell culture supernate samples.
Validate diluents for specific sample types prior to use. Wear eye, hand, face, and clothing protection when using this material. It is recommended that all standards and samples be assayed in duplicate. Avoid microbial contamination of reagents and buffers.
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