名称：原装正品| Rat TNF-alpha DuoSet
TECHNICAL HINTS AND LIMITATIONS
If the standard curve appears suppressed, consider evaluating a different preparation of BSA. A standard curve should be generated for each set of samples assayed. Remove any remaining Wash Buffer by inverting the plate and blotting it against clean paper towels. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. Use a fresh reagent reservoir and pipette tips for each step.
The Stop Solution suggested for use with this kit is an acid solution. It is recommended that all standards and samples be assayed in duplicate. Avoid microbial contamination of reagents and buffers. Buffers containing a large quantity of protein should be made under sterile conditions and stored at 2 - 8° C or be prepared fresh daily. Wear eye, hand, face, and clothing protection when using this material. This may interfere with the sensitivity of the assay.
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