T4 Endonuclease V/Buffer_中国教育装备采购网

上海叶舟生物科技有限公司

 T4 Endonuclease V/Buffer
  • T4 Endonuclease V/Buffer
  • T4 Endonuclease V/Buffer

    参考价格: ¥3650.00
    品  牌:R&D
    产品型号:4055-100-EB (100000 UN)
    所在地区:上海
    上架时间:2012年09月27日
    留言咨询
    与企业取得联系时请告知该信息获取自中国教育装备采购网
    我要分享
    详细说明

    T4 Endonuclease V/Buffer
    名称:原装正品| T4 Endonuclease V/Buffer
    货号:4055-100-EB
    产地:R&D
    规格:100000 UN
    价格:3650
    Storage Conditions: Store at 4°C. Do not freeze enzyme.
    Source: Purified from E. coli containing a recombinant plasmid harboring the T4 phage denV gene. T4 Endonuclease V is a DNA glycosylase of MW 16 kDa with an associated AP lyase activity that is specific for the cis-syn isomer of cyclobutane pyrimidine dimers induced by UV irradiation. This highly processive enzyme cleaves the glycosyl bond, 5’ to the pyrimidine dimer,
    Description:  via nucleophilic attack of the alpha amino group of Thr-2, forming a series of protein-DNA imino intermediates.1,2 The associated AP lyase activity results in cleavage of the phosphodiester bond 3’ to the AP site, thus generating a 3’ unsaturated aldehyde and a 5’ phosphate (Figure 1). One Unit is the amount of enzyme required to completely relax 250 ng of a UV-irradiated supercoiled plasmid in 30 minutes at 37°C. This site is then incised on the 5’ side by an AP endonuclease, generating an appropriate substrate for resynthesis and DNA repair.
    If you want to learn more about the product information, welcome to inquire!