名称：原装正品| T4 Endonuclease V/Buffer
Storage Conditions: Store at 4°C. Do not freeze enzyme.
Source: Purified from E. coli containing a recombinant plasmid harboring the T4 phage denV gene. T4 Endonuclease V is a DNA glycosylase of MW 16 kDa with an associated AP lyase activity that is specific for the cis-syn isomer of cyclobutane pyrimidine dimers induced by UV irradiation. This highly processive enzyme cleaves the glycosyl bond, 5’ to the pyrimidine dimer,
Description: via nucleophilic attack of the alpha amino group of Thr-2, forming a series of protein-DNA imino intermediates.1,2 The associated AP lyase activity results in cleavage of the phosphodiester bond 3’ to the AP site, thus generating a 3’ unsaturated aldehyde and a 5’ phosphate (Figure 1). One Unit is the amount of enzyme required to completely relax 250 ng of a UV-irradiated supercoiled plasmid in 30 minutes at 37°C. This site is then incised on the 5’ side by an AP endonuclease, generating an appropriate substrate for resynthesis and DNA repair.
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