名称：原装精品| h/m/rTotal TC-PTP DuoSet IC
Store the unopened kit at 2 - 8° C. Do not use past kit expiration date.
TECHNICAL HINTS AND LIMITATIONS
Individual results may vary due to differences in technique, plasticware and water sources. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. The data may be linearized by plotting the log of the TC-PTP concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. Use a fresh reagent reservoir and pipette tips for each step. It is recommended that all standards and samples be assayed in duplicate.
CALCULATION OF RESULTS
Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Buffers containing protein should be made under aseptic conditions and stored at 2 - 8° C or be prepared fresh daily. This DuoSet IC ELISA should not be used beyond the expiration date on the kit label. Avoid microbial contamination of reagents and buffers. Results may be normalized to total protein or cell number.
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