名称：Blood & Cell Culture DNA Maxi Kit
For isolation of up to 500 µg high-molecular-weight DNA from blood and cultured cells: Convenient, parallel processing of multiple samples. Reliable isolation of DNA up to 150 kb in size. No phenol or chloroform extractions.
Analysis of gene targeting. Screening of transgenic animals. DNA fingerprinting studies. Southern blotting. QIAGEN Genomic-tips, included in Blood & Cell Culture DNA Kits, use unique QIAGEN anion-exchange technology to purify high-molecular-weight DNA from a wide range of biological samples without phenol or chloroform. PCR amplification. The QIAGEN Genomic-tip procedure is very gentle and results in negligible DNA shearing. DNA purified with QIAGEN Genomic-tips is sized up to 150 kb with an average length of 50–100 kb.
The DNA is free of all contaminants such as RNA, protein, and metabolites, and has A260/A280 ratios between 1.7 and 1.9, making it well suited for use in the following applications: RFLP analysis. Lysis buffers are optimized for different sample types and provide immediate denaturation of proteins such as nucleases, histones, and DNA-binding proteins, as well as potentially infectious viral particles.
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