名称：现货供应|p53 (1C12) Mouse mAb
Source / Purification
Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser20 of human p53. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage .
Specificity / Sensitivity
DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 (1C12) Mouse mAb detects endogenous levels of total p53 protein. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation.