名称：原装进口| PathScan Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit
Kit should be stored at 4°C with the exception of Cell Lysis Buffer (10X), which is stored at –20°C (packaged separately).
Specificity / Sensitivity
CSTs PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit detects endogenous levels of Phospho-VEGFR-2 protein. HRP-linked anti-rabbit antibody (#7074) is then used to recognize the bound detection antibody.
However, the level of total VEGFR-2 (phospho and non-phospho), detected by PathScan® Total VEGFR-2 Sandwich ELISA Kit #7340, remains unchanged (Figure 1). Following extensive washing, a phospho-VEGFR-2 Rabbit mAb is added to detect the captured phospho-VEGFR-2 protein.
A VEGFR-2 Mouse mAb has been coated onto the microwells. After incubation with cell lysates, both nonphospho- and phospho-VEGFR-2 proteins are captured by the coated antibody. Using this Sandwich ELISA Kit #7335, a significant induction of phospho-VEGFR-2 in HUVE* cells treated with VEGF can be detected.