名称：原装进口| SimpleChIP Mouse GAPDH Intron 2 Primers
Directions For Use
The GAPDH gene is actively transcribed in all cell types and is highly enriched for histone modifications associated with active transcription, such as histone H3 Lys4 tri-methylation and general histone acetylation.
PCR reactions should be performed in duplicate and should include a tube with no DNA to control for contamination, and a serial dilution of a 2% total input chromatin DNA (undiluted, 1:5, 1:25, 1:125), which is used to create a standard curve and determine amplification efficiency.
Label the appropriate number of PCR tubes or PCR plates compatible with the model of real-time PCR machine to be used.
Add 2 µl of the appropriate ChIP DNA sample to each tube or well of the PCR plate. Prepare a master PCR reaction mix as described below. Add enough reagents for two extra reactions to account for loss of volume. Add 18 µl of the master PCR reaction mix to each PCR reaction tube or well of the PCR plate.
The intron 2 region shows very low levels of histone modifications associated with heterochromatin, such as histone H3 Lys9 or Lys27 tri-methylation. These primers can be used to amplify DNA that has been isolated using chromatin immunoprecipitation (ChIP). SimpleChIP® Mouse GAPDH Intron 2 Primers contain a mix of forward and reverse PCR primers that are specific to intron 2 of the mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene.