名称：原装进口| SimpleChIP Human α Satellite Repeat Primers
Directions For Use
Add 2 µl of the appropriate ChIP DNA sample to each tube or well of the PCR plate. Add enough reagents for two extra reactions to account for loss of volume. Analyze quantitative PCR results using software provided with the real-time PCR machine.
SimpleChIP® primers have been optimized for amplification of ChIP-isolated DNA using real-time quantitative PCR and provide important positive and negative controls that can be used to confirm a successful ChIP experiment. Prepare a master PCR reaction mix as described below. Add 18 µl of the master PCR reaction mix to each PCR reaction tube or well of the PCR plate.
SimpleChIP® Human α Satellite Repeat Primers contain a mix of forward and reverse PCR primers that are specific to the human α satellite repeat element. Reactivity Key: H=Human.
The human α satellite repeat element is found in pericentromeric regions of chromosomes and shows hallmarks of heterochromatin including histone H3 Lys9 di- and tri-methylation, as well as HP1 binding. Applications Key: ChIP=Chromatin IP. These primers can be used to amplify DNA that has been isolated using chromatin immunoprecipitation (ChIP).