名称：原装进口| SimpleChIP Mouse RPL30 Intron 2 Primers
Directions For Use
Add 2 µl of the appropriate ChIP DNA sample to each tube or well of the PCR plate. Add 18 µl of the master PCR reaction mix to each PCR reaction tube or well of the PCR plate. Analyze quantitative PCR results using software provided with the real-time PCR machine. Prepare a master PCR reaction mix as described below. Add enough reagents for two extra reactions to account for loss of volume.
This gene promoter shows very low levels of histone modifications associated with heterochromatin, such as histone H3 Lys9 and Lys27 tri-methylation. Reactivity Key: M=Mouse. SimpleChIP® Mouse RPL30 Intron 2 Primers contain a mix of forward and reverse PCR primers that are specific to intron 2 of the mouse RPL30 gene.
The RPL30 gene is actively transcribed in all cell types and its promoter is highly enriched for histone modifications associated with active transcription, such as histone H3 Lys4 tri-methylation and general histone acetylation. Applications Key: ChIP=Chromatin IP. These primers can be used to amplify DNA that has been isolated using chromatin immunoprecipitation (ChIP).
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