名称：原装进口| Immunization Grade Rat Type II Collagen
STORAGE TEMPERATURE: 4°C in the dark.
STABILITY: 2 years.
PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans.
SOURCE: Rat sternal cartilage.
QUANTITY: 5 mg.
Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose and Na2HPO4). Soluble in acidic buffer (maximum 4 mg/ml), but difficult to dissolve in neutral buffer. In order to dissolve this product in a neutral buffer.
Can be used for various purposes, such as a standard for collagen analysis by SDS-gel, substrate for MMP-1, MMP-8 and MMP-13, and as an antigen for immunizing animals for the induction of collagen-induced arthritis (CIA) in certain species and strains of experimental animals. use 0.01M or 0.05M acetic acid to dissolve the collagen at 1-4 mg/ml and then add this collagen solution to a desired neutral buffer such as 2-10X Tris-NaCl (the final concentration of NaCl: 0.15-0.2M) or dialyze against 1X neutral buffer at 4°C.
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