名称：原装进口| Immunization Grade Sheep Type II Collagen
STORAGE TEMPERATURE: 4°C in the dark.
STABILITY: 1 year.
PURITY: >95% by SDS-PAGE, free of pepsin and proteoglycans (Figure 1).
SOURCE: Sheep articular cartilage.
Highly purifi ed type II collagen (treated with 3M guanidine, DEAE-cellulose and Na2HPO4). In order to dissolve this product in a neutral buffer, use 0.01M or 0.05M acetic acid to dissolve the collagen at 1-4 mg/ml and then add this collagen solution to a desired neutral buffer such as 2-10X Tris-NaCl (the fi nal concentration of NaCl: 0.15-0.2M) or dialyze against 1X neutral
Can be used for various purposes, such as a standard for collagen analysis by SDS-gel, substrate for MMP-1, MMP-8, and MMP-13, and as an antigen for immunizing animals for developing anti-sheep type II collagen. Soluble in acidic buffer (maximum 4 mg/ml), but diffi cult to dissolve in neutral buffer. buffer at 4°C.
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