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Cholera Toxin|霍乱毒素|现货

Cholera Toxin|霍乱毒素|现货

参考价格:¥1985.00
品  牌:Sigma
产品型号:C8052 (0.5MG)
所在地区:华东 上海
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Cholera Toxin|霍乱毒素|现货
Cholera Toxin|霍乱毒素|现货供应
产地:Sigma
货号:C8052
规格:0.5MG
别名: Cholera enterotoxin, Cholergen
CAS号: 9012-63-9
纯度 ~95% (SDS-PAGE)
保存温度: 2-8°C
Package size based on protein content.
产品说明:
When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
Unit Definition
One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthine (IBMX).
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA
Biochem/physiol Actions
Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12. Bacteria, Bioactive Small Molecules, C-CH, Cell Biology, Cell Signaling and Neuroscience, G Protein Function, G Proteins and Cyclic Nucleotides, Reagents, Toxins and Venoms
Quality Level PREMIUM
form lyophilized powder
composition Protein, ~5% Lowry-TCA 
solubility H2O: soluble10 mg/mL