规格: 20 X 50UL
The BL21-AI? E. coli strain offers the tightest regulation of expression for production of toxic proteins using the T7 promoter. This strain was constructed by inserting a chromosomal copy of the T7 RNA polymerase gene (T7 RNAP) under control of the arabinose-inducible araBAD promoter (1). The araBAD promoter tightly regulates expression of the T7 RNAP, and hence expression of the gene of interest in
T7-mediated expression systems. Upon induction, the araBAD promoter exhibits strong expression, resulting in high-level protein production (Figures 1 and 2). In addition, expression levels can be adjusted, or reduced, to improve protein folding and solubility. The BL21-AI? Chemically Competent Cells are supplied in the convenient, single-use One Shot? format.