上海浩然生物技术有限公司

 Porablot NCP 原装
  • Porablot NCP 原装
  • Porablot NCP 原装

    参考价格: ¥2093.00
    品  牌:MN
    产品型号:741283
    所在地区:上海
    上架时间:2018年11月01日
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    详细说明

    产品: MN
    货号: 741283
    规格: (82 mm, 50 discs)
    名称: Porablot NCP
    Membrane material 100 % nitrocellulose
    Membrane character Hydrophilic
    Pore size 0.45 µm
    Binding capacity 100 µg/cm2
    Applications
    • Southern, Northern, and Western blotting, colony and plaque transfer, dot blotting
    • Also available with a supporting tissue, see Porablot NCL
    Membrane filters of high purity nitrocellulose are the classical transfer medium for binding proteins and nucleic acids. The range of application includes Southern, Northern and Western blotting as well as colony, plaque transfer and dot blotting. Although the binding mechanism is not understood in all details, it is assumed that the macromolecules are bound via hydrophobic interaction and hydrogen bonding. Porablot NCP membranes consist of 100 % nitrocellulose and are supplied with a pore size of 0.45 μm. With a binding capacity of 100 μg/cm2 this membrane is well suited for protein blotting. The hydrophilic character of this membrane ensures good contact with the gel during botting. Additionally, nitrocellulose can be easily blocked, for example with casein or albumin. Due to the low background, all conventional detection methods, such as antigen/antibody, autoradiography, biotin labelling and chemiluminescence, yield good results.