产品名称：Detector Random Primer DNA Biotinylation Kit
Detection method: DNA probe biotinylation
Stability: 1 year
Biotinylate DNA in as little as 1 hour with maximum template amplification.
Quantitation standard allows for determination of probe specific activity.
Large quantities of biotinylated probes can be generated from a small amount of template.
The DetectorTM Random Primer DNA Biotinylation Kit provides a method for biotinylating DNA probes through incorporation of biotin-dCTP during random primer extension. A template with a minimum size of 300 base pairs is required. Only 100 ng template DNA is required per reaction. Exo- Klenow Polymerase permits longer labeling reactions without degradation of nucleotides. DNA probes are stable for up to 1 year without loss of activity. Probes labeled with biotin using the random primer method can be used on Southern blots, Northern blots, dot blots, in situ hybridization, colony lifts, and plaque hybridizations. Sufficient reagents are provided to perform 30 labeling reactions following the enclosed protocol.
1. 2.5X Random Primer Solution 2. 10X dNTP mixture 3. Exo- Klenow DNA Polymerase 4. Control Template DNA 5. DEPC Treated Water 6. Biotinylated Quantitation Standard 7. Stop Buffer