Inhibitor of deSUMOylating enzymes (SENPs). SUMO-2 aldehyde is a highly specific inhibitor of SENP enzymes and has utility in the stabilisation of SUMO-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts.
Short Term Storage: +4°C
Long Term Storage: +4°C
Handling: Do not freeze.
Formulation: Liquid. In 0.15M HCl.
Purity: ≥95% (HPLC)
Source: Produced in E. coli cells. Derivatised chemically, in vitro.
Use/Stability: Soluble and stable in aqueous solution at pH ≤7.0. Avoid presence of amino-containing compounds. Do not lyophilise. Do not neutralise until immediately prior to use.Stable for at least 2 months after receipt when stored at +4°C.
Miscellaneous/General: SUMO proteases (SENPs) contribute to three distinct activities in the cell, processing SUMO precursors to generate mature SUMO, depolymerization of SUMO chains, and deconjugation of SUMO modified proteins. Cleavage occurs after the conserved SUMO Gly-Gly motif either at the scissile peptide bond during processing or at the scissile isopeptide bond during depolymerization and deconjugation. The six human SUMO specific SENP enzymes (1-3, 5-7), have been shown to participate in non-redundant cellular functions such as HIF1-α stabilization during hypoxia, mitosis and cytokinesis.