规格: 1 MG
名称: CellTracker™ Green CMFDA (5-Chloromethylfluorescein Diacetate),
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate safe handling of potentially pathogenic samples, and to permit signal amplification in cases where the anti-dye antibody is available. The signal from CellTracker™ probes is typically monitored using fluorescence microscopy, and we offer CellTracker™ probes in several fluorescent colors to fit into multicolor analyses.
CellTracker™ Dyes Specifications:
• Label (Ex/Em): CellTracker™ Green CMFDA (492/View More517 nm)
• Requires enzymatic cleavage to activate fluorescence: Yes
• Lyophilized product should be dissolved using DMSO and then added to culture medium to make a working solution
• Not compatible with amine and thiol-containing buffers
Applications for CellTracker™ Dyes
CellTracker™ dyes represent a major breakthrough in the cellular retention of vital probes and are excellent tools for long-term studies of normal and transformed cells in culture and for investigating cell viability and cytotoxicity, transplantation, and cell fusion.
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.