规格: 5 ML
名称: Dynabeads® Mouse DC Enrichment Kit
Use this kit to enrich viable mouse dendritic cells (DC’s) from spleen/lymph node cells by negative isolation. The kit depletes T cells, mIgM+ B cells, NK cells, erythrocytes and most granulocytes. Highly enriched mouse DC’s are bead and antibody-free, and can be used in any functional assay. The cells can be further sorted to obtain high purity of specific DC sub-populations.
Obtain high recovery (80-100%) of viable Lin-CD11c+ DC’s of all sup-populations, including DC’s positive for CD4, CD8α, B220 and CD19.
Substantial reduction in sorting time and volume compared to sorting only, thus saving money in addition.
Pool cells/tissue for easy sample scale –up
An antibody mix towards the non-dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched mouse DC’s can be directly sorted into the sup-population of choice or used in any other application.
Spleen or lymph node cells.
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.