上海浩然生物技术有限公司

 FLUO-3- AM FLUOROPURE(TM 原装正品
  • FLUO-3- AM FLUOROPURE(TM 原装正品
  • FLUO-3- AM FLUOROPURE(TM 原装正品

    参考价格: ¥3355.00
    品  牌:invitrogen
    产品型号:F23915
    所在地区:上海
    上架时间:2018年12月11日
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    FLUO-3- AM FLUOROPURE(TM 原装正品
    产品: invitrogen
    货号: F23915
    规格: 10 X 50 UG
    名称: Fluo-3, AM *FluoroPure™ grade* *Special Packaging*
    Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.
    Calcium Indicator (AM Ester) Specifications:
    • Label (Ex/Em of Ca2+–bound form): Fluo-3 (506/526 nm)
    • Fluorescence intensity increase upon binding Ca2+: >100 fold
    • Kd for Ca2+ in buffer: ~335 nM
    • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
    More Choices for Fluorescent Calcium Indicators
    We offer a large selection of Molecular Probes® calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes® Handbook.
    Using TPEN to Control Heavy Metal Cations
    In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.
    For Research Use Only. Not for any animal or human therapeutic or diagnostic use.