FXR COACTIVATOR ASSAY 原装正品_中国教育装备采购网

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 FXR COACTIVATOR ASSAY 原装正品
  • FXR COACTIVATOR ASSAY 原装正品
  • FXR COACTIVATOR ASSAY 原装正品

    参考价格: ¥12660.00
    品  牌:invitrogen
    产品型号:PV4833
    所在地区:上海
    上架时间:2018年08月16日
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    FXR COACTIVATOR ASSAY 原装正品
    产品: invitrogen
    货号: PV4833
    规格: 800 X 20UL
    名称: LanthaScreen™ TR-FRET FXR Coactivator Assay Kit
    The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format.
    To assay:
    When running the LanthaScreen™ TR-FRET FXR coactivator assay in agonist mode, FXR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the FXR-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide
    The LanthaScreen™ TR-FRET FXR Coactivator Assay Kit contains FXR-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers.
    Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
    For Research Use Only. Not for any animal or human therapeutic or diagnostic use.