名称: Blood RNA Kit(50)
The kit adopts silica gel column purification method, suitable for less than 1.5ml from fresh or frozen storage of anticoagulated blood samples in rapid and simple method for extracting total RNA. General1ml in whole blood samples can be purified from1 -5 g RNA, extraction process does not need to use phenol chloroform and other harmful organic extraction and time-consuming isopropanol precipitation. The method can completely remove the downstream effects of experiments with various inhibitory factor, purification of the RNA can be directly used for RT-PCR, Northern hybrid, in vitro translation and nucleic acid protection. At the same time the reagent box is also suitable for from cultured cells, tissue and RNA samples of the virus RNA extraction. Blood red cell lysate ( ERL ) from red cell lysis, joined the TRK Lysis Buffer cleavage of leukocytes, lysate by homogenate column filtering to remove impurities, with 70% ethanol modulate the binding conditions, the column adsorption of RNA, after three step rapid washing out the impurities dissolved in water, the last RNA DEPC.
Product characteristics and advantages
Rapid within 60min completed an experiment
Safety without phenol chloroform and other organic extraction
Reliable has the advantages of simple operation, each time can obtain ideal effect
Stability silica gel column combination mode, yield stability
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.