规格: 100 UN
名称: DNA Polymerase I, Large (Klenow) Fragment
DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase that Polymerase I, but it contains the 3´ 5´ exodeoxyribonuclease activity.
Performance and Quality Testing: SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.
Applications: Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.
Source: Purified from E. coli expressing the Klenow fragment on a plasmid.
Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.