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 LENTI HIPERFOM T-REX EXPSYST 正品原装
  • LENTI HIPERFOM T-REX EXPSYST 正品原装
  • LENTI HIPERFOM T-REX EXPSYST 正品原装

    参考价格: ¥36750.00
    品  牌:invitrogen
    产品型号:A11141
    所在地区:上海
    上架时间:2018年06月04日
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    LENTI HIPERFOM T-REX EXPSYST 正品原装
    产品: invitrogen
    货号: A11141
    规格: 20 reactions
    名称: ViraPower™ HiPerform™ T-REx™ Gateway® Expression System
    The ViraPower™ HiPerform™ T-REx™ Gateway® Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
    Key Features
    • WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, increases transgene expression and cPPT (central Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
    • Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
    • Blasticidin selection marker for stable selection under control of SV40 promoter
    Advantages
    • Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
    • Easy recombination-based cloning using Gateway® technology
    • Stable, long-term, tetracycline-regulated expression
    • Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems
    For Research Use Only. Not for any animal or human therapeutic or diagnostic use.