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 LXR ALPHA COACTIVATOR ASSAY KIT 原装正品
  • LXR ALPHA COACTIVATOR ASSAY KIT 原装正品
  • LXR ALPHA COACTIVATOR ASSAY KIT 原装正品

    参考价格: ¥12810.00
    品  牌:invitrogen
    产品型号:PV4655
    所在地区:上海
    上架时间:2018年08月28日
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    LXR ALPHA COACTIVATOR ASSAY KIT 原装正品
    产品: invitrogen
    货号: PV4655
    规格: 800 X 20 UL ASSAYS
    名称: LanthaScreen™ TR-FRET LXR Alpha Coactivator Assay Kit
    The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
    Contents and Storage:
    The LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit contains LXR alpha-LBD (GST) protein, fluorescently labeled TRAP220/DRIP-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
    In the LanthaScreen™ TR-FRET LXR alpha Coactivator Assay, LXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
    For Research Use Only. Not for any animal or human therapeutic or diagnostic use.