上海浩然生物技术有限公司

第6年
LXR BETA COACTIVATOR ASSAY KIT 原装正品

LXR BETA COACTIVATOR ASSAY KIT 原装正品

参考价格:¥12810.00
品  牌:invitrogen
产品型号:PV4658
所在地区:上海
留言咨询
  QQ交谈
与企业取得联系时请告知该信息获取自中国教育装备采购网
我要分享
详细说明

LXR BETA COACTIVATOR ASSAY KIT 原装正品
产品: invitrogen
货号: PV4658
规格: 800 X 20 UL ASSAYS
名称: LanthaScreen™ TR-FRET LXR Beta Coactivator Assay Kit
The LanthaScreen™ TR-FRET LXR (liver X receptor) beta Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR beta ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR beta ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
Contents and Storage:
The LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit contains LXR beta-LBD (GST) protein, fluorescently labeled D22 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
How it works:
In the LanthaScreen™ TR-FRET LXR beta Coactivator Assay, LXR beta-LBD is added to ligand test compounds followed by the addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide .
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.