上海浩然生物技术有限公司

4008190228-0529
 T4 POLYNUCLEOTIDE KINASE 原装正品
  • T4 POLYNUCLEOTIDE KINASE 原装正品
  • T4 POLYNUCLEOTIDE KINASE 原装正品

    参考价格: ¥2990.00
    品  牌:invitrogen
    产品型号:18004028
    所在地区:上海
    上架时间:2018年08月28日
    留言咨询
    4008190228-0529
    与企业取得联系时请告知该信息获取自中国教育装备采购网
    我要分享
    详细说明

    T4 POLYNUCLEOTIDE KINASE 原装正品
    产地: invitrogen
    货号: 18004028
    规格: 1000 UN
    名称: T4 Polynucleotide Kinase
    T4 Polynucleotide Kinase catalyzes the transfer of the γ -phosphate of ATP to the 5´ hydroxyl terminus of DNA or RNA.
    A T4 Polynucleotide Kinase Technical Bulletin is available.
    Source: Purified from E. coli expressing the T4 polynucleotide kinase gene in a λ vector.
    Applications: 5´ end-labeling of DNA or RNA (1). Phosphorylation of synthetic linkers (1).
    Performance and Quality Testing: Ribonuclease, endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease, and phosphatase assays;
    labeling efficiency tested.
    Unit Reaction Conditions: 70 mM Tris-HCl (pH 7.6), 0.1 M KCl, 10 mM MgCl2 , 1 mM 2-mercaptoethanol, 0.3 mM [γ-32P]ATP,
    0.38 mg/ml micrococcal nuclease-treated DNA, and enzyme in 150 µl for 30 min. at 37°C.
    Unit Definition: One unit transfers 1 nmol of phosphate of ATP to the 5´ hydroxyl termini of micrococcal nuclease-treated DNA in
    30 min. at 37°C.
    For Research Use Only. Not for any animal or human therapeutic or diagnostic use.