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 Pierce TMB-Blotting Substrate Solution? 原装正品
  • Pierce TMB-Blotting Substrate Solution? 原装正品
  • Pierce TMB-Blotting Substrate Solution? 原装正品

    参考价格: ¥1386.00
    品  牌:Pierce
    产品型号:34018
    所在地区:上海
    上架时间:2018年11月01日
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    详细说明

    产地: Pierce
    货号: 34018
    规格: 250ml
    名称: Pierce TMB-Blotting Substrate Solution
    Thermo Scientific Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods.
    TMB (3,3,5,5-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.
    Requires:
    For best results, use TMB-Blotting Substrate with blots that have been blocked with Thermo Scientific SuperBlock Blocking Buffers for Blotting (Part No. 37517, 37537)
    Highlights:
    HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media
    Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates
    Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use
    Complete – does not require other buffers or addition of hydrogen peroxide
    For Research Use Only. Not for any animal or human therapeutic or diagnostic use.