规格: 50 tests
名称: Epstein Barr virus Probe ISH Kit
This kit comprises proteinase K, detection probe, (negative) control probe, anti-fluorescein detection system, unlabeled control tissue sections, and 3 aminopropyltriethoxysilane (APES) treated slides. Each kit provides reagents sufficient for 50 tests.
The Epstein-Barr virus (EBV) probe demonstrates cells latently infected with EBV. The probe hybridizes to abundantly expressed Epstein-Barr virus-encoded RNA (EBER) transcripts which are concentrated in the nuclei of latently infected cells. These transcripts are thought to block the activation of dsRNA-dependent eukaryotic initiation factor 2a (elF-2a) protein kinase DAI. In the absence of EBER, elF-2a inhibits cellular protein synthesis. The EBER molecules, therefore, promote both host cell survival and viral growth.
EBV infection is associated with a variety of pathological conditions. The virus has been demonstrated by in situ hybridization in infectious mononucleosis, Burkitt’s lymphoma, the Reed Sternberg cells of Hodgkin’s disease and in nasopharyngeal carcinoma. In HIV infection EBV has also been demonstrated in primary CNS lymphomas and oral hairy leukoplakia lesions.