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 GenMute siRNA Transfection Reagent for Jurkat Cell 原装正品
  • GenMute siRNA Transfection Reagent for Jurkat Cell 原装正品
  • GenMute siRNA Transfection Reagent for Jurkat Cell 原装正品

    参考价格: ¥1980.00
    品  牌:SignaGen
    产品型号:SL100568-JURKAT (1.0 ml)
    所在地区:上海
    上架时间:2018年08月28日
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    GenMute siRNA Transfection Reagent for Jurkat Cell 原装正品
    产地: SignaGen
    货号: SL100568-JURKAT
    规格: 1.0 ml
    名称: GenMute siRNA Transfection Reagent for Jurkat Cell 原装正品
    Size & content:
    - GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 10 pmoles siRNA or miRNA mimics in 24-well plate
    - GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
    Description:
    GenMute™ siRNA Transfection Reagent for Jurkat Cell is pre-optimized for transfecting siRNA to Jurkat cells. Jurkat cells are an immortalized line of T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.
    Summary of Optimal Transfection Conditions:
    Cell culture medium      DMEM with 4.5 g/L glucose, 10% FBS
    Diluent for siRNA and GenMute™ Reagent    1xGenMute™ Transfection Buffer
    Incubation Time to Form GenMute™/siRNA Complex   ~15 minutes at RT
    Confluency of cell on the day of transfection   ~50%
    Optimal siRNA concentration     30 nM
    Maximal Silencing Efficiency      48 hours