上海浩然生物技术有限公司

 GenJet In Vitro DNA Tranfection Reagent for L929 Cell 原装正品
  • GenJet In Vitro DNA Tranfection Reagent for L929 Cell 原装正品
  • GenJet In Vitro DNA Tranfection Reagent for L929 Cell 原装正品

    参考价格: ¥1680.00
    品  牌:SignaGen
    产品型号:SL100489-L929 (1.0 ml)
    所在地区:上海
    上架时间:2018年12月11日
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    GenJet In Vitro DNA Tranfection Reagent for L929 Cell 原装正品
    产地: SignaGen
    货号: SL100489-L929
    规格: 1.0 ml
    名称: GenJet In Vitro DNA Tranfection Reagent for L929 Cell 原装正品
    Description
    GenJet™ DNA In Vitro Tranfection Reagent for L929 cell is pre-optimized for transfecting L929 cells. L929 is a murine aneuploid fibrosarcoma cell line which is often used to assay TNF-alpha and TNF-beta. Treatment with TNF initiates apoptosis and subsequent cell death.  The level of detection is on the order of 50 pg/mL. Another cell line which is approximately 5-10-fold more sensitive is WEHI-164. Some L929 subclones have been developed that allow detection of TNF at concentrations of approximately 30 pmol/L (approximately 500 fg/mL). Sublines that are resistant to TNF have been established also.
    Summary of Optimal Transfection Conditions:
    Confluence on the day of transfection  ~80%
    Cell culture conditions    DMEM with 4.5 g/L glucose, 10% FBS
    GenJet™ (µl) : DNA (µg) Ratio   3:1
    Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
    Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
    Presence of Serum/Antibiotics during   Yes
    Transfection 
    Change Medium After Transfection  Yes, ~5 Hours After
    Maximal Efficiency     48 hours

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