规格: 500 µg
名称: Apolipoprotein A-I human 原装正品
Key Applications: Positive Control
Product Overview: High Density Lipoprotein (HDL) isolated by ultracentrifugation are delipidated. Apo A-I is purified by column chromatography in 6M urea (Schonfeld et al. 1977). Purity is determined by quantitative densitometric of SDS polyacrylamide gels (Laemlli 1970). Protein is determined by the Pierce BCA method (which was found to be comparable to the Lowry method) with BSA as the standard.
Brand Family: Chemicon
Source: Normal human plasma, fresh, non-frozen. Tested negative for HIV-1, HIV-2, HCV and HBc antibodies and HBsAg.
Presentation: Liquid in 10 mM NH4HCO3, pH 7.4. Non-sterile, no preservatives.
UniProt Number: P02647
Storage Conditions: Maintain lyophilized material at < -20°C for up to six months. Maintain reconstituted Apo A-I at +4°C up to 4 weeks. Freezing solutions of apolipoproteins in the absence of denaturants may favor increased self-association or aggregation.