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 TaKaRa Taq Polymerase 原装正品
  • TaKaRa Taq Polymerase 原装正品
  • TaKaRa Taq Polymerase 原装正品

    参考价格: ¥1579.00
    品  牌:millipore
    产品型号:R001A (250 units)
    所在地区:上海
    上架时间:2018年06月04日
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    TaKaRa Taq Polymerase 原装正品
    产地: millipore
    货号: R001A
    规格: 250 units
    价格(元):1579
    名称: TaKaRa Taq Polymerase 原装正品
    Key Applications: DNA Amplification
    Application Notes: DNA amplification by Polyermerase Chain Reaction (PCR)
    DNA sequencing
    PCR condition (an example)
    When amplifying 1 kbp DNA fragment
    30 cycles of : 94°C 30sec, 55°C 30sec, 72°C 1min. Note: Denaturation condition varies depending on a used thermal cycler and tube. It is recommednded for 10-30sec at 94°C , or 1-10sec at 98°C.
    General reaction mixture for PCR (total 100μL)
    TaKaRa™ (5units/μL) 0.5μL
    10X PCR Buffer 10μL
    dNTP Mixture (2.5mM each) 8μL
    Template <1μg
    Primer 1 20 - 100 pmol (final conc. 0.2 - 1.0 μM)
    Primer 2 20 - 100 pmol (final conc. 0.2 - 1.0 μM)
    Sterilized distilled water up to 100μL
    Storage Conditions: Store at -20°C.