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TaKaRa Taq Polymerase 原装正品

TaKaRa Taq Polymerase 原装正品

参考价格:¥1579.00
品  牌:millipore
产品型号:R001A (250 units)
所在地区:上海
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TaKaRa Taq Polymerase 原装正品
产地: millipore
货号: R001A
规格: 250 units
价格(元):1579
名称: TaKaRa Taq Polymerase 原装正品
Key Applications: DNA Amplification
Application Notes: DNA amplification by Polyermerase Chain Reaction (PCR)
DNA sequencing
PCR condition (an example)
When amplifying 1 kbp DNA fragment
30 cycles of : 94°C 30sec, 55°C 30sec, 72°C 1min. Note: Denaturation condition varies depending on a used thermal cycler and tube. It is recommednded for 10-30sec at 94°C , or 1-10sec at 98°C.
General reaction mixture for PCR (total 100μL)
TaKaRa™ (5units/μL) 0.5μL
10X PCR Buffer 10μL
dNTP Mixture (2.5mM each) 8μL
Template <1μg
Primer 1 20 - 100 pmol (final conc. 0.2 - 1.0 μM)
Primer 2 20 - 100 pmol (final conc. 0.2 - 1.0 μM)
Sterilized distilled water up to 100μL
Storage Conditions: Store at -20°C.