规格: 40 assays
名称: ApopTag Peroxidase In Situ Apoptosis Detection Kit 原装正品
Application Notes: INTRODUCTION
ApopTag® In Situ Apoptosis Detection Kits label apoptotic cells in research samples by modifying DNA fragments utilizing terminal deoxynucleotidyl transferase (TdT) for detection of apoptotic cells by specific staining.
The ApopTag® Peroxidase Kit has been qualified for use in histochemical and cytochemical staining of the following specimens: formalin-fixed, paraffin-embedded tissues, cryostat sections, cell suspensions, cytospins, and cell cultures. Whole mount-methods have been developed (34, 45).
The staining specificity of the ApopTag® Peroxidase Kit has been demonstrated by Chemicon and many other laboratories. Chemicon has tested many types of model cell and tissue systems, including: (a) human prostate, thymus, and large intestine (in-house data); (b) rat ventral prostate post-castration (21), (c) rat thymus lymphocytes treated in vitro with dexamethasone (3, 13), (d) 14-day mouse embryo limbs (1) and (e) rat mammary gland in regression after weaning (36). In the thymocyte and prostate models, agarose gel electrophoresis was used to assess the amount of DNA laddering, which peaked coincidentally with the maximum percentage of stained cells. Numerous journal publications from laboratories worldwide have established the usefulness of ApopTag® Kits. (See Sec. V. References, Publications Citing ApopTag® Kits).
Components: Equilibration Buffer: 3.0 mL -15°C to -25°C
Reaction Buffer 2.0 mL -15°C to -25°C
TdT Enzyme 0.64 mL -15°C to -25°C
Stop/Wash Buffer 20 mL -15°C to -25°C
Anti-Digoxigenin-Peroxidase* 3.0 mL 2°C to 8°C
Plastic Coverslips 100 ea. Room Temp.
Note: Separate purchase of DAB (Peroxidase Substrate) is required. It is not supplied with this kit.
Number of samples per kit: Sufficient materials are provided to stain 40 tissue specimens of approximately 5 cm2 each when used according to instructions. Reaction Buffer will be fully consumed before other reagents when kits are used for slide-mounted specimens.
Storage Conditions: Store the kit at -15°C to -25°C until the first use. After the first use, if the kit will be used within three months, store the TdT Enzyme (90418) at -15°C to -25°C and store the remaining components at 2°C to 8°C.