规格: 5 ea
名称: ApopTag Positive Control Slides rat mammary gland 4d post weaning 原装正品
Application Notes: Interpretation of Staining
These positive control slides of female rat mammary tissue 4-days post weaning show only 1-2% of the cells being apoptotic. Microscopic inspection is usually required to confirm proper kit activity.
Stain is generally localized to chromatin, and is intensely colored. The localization and sensitivity to be expected with the ApopTag® Peroxidase (S7100, S7101) or ApopTag® Fluorescein (S7110, S7111, S7160, S7165) Kits are similar. Positive objects vary in size, from intact nuclei to much smaller apoptotic bodies, and in shape, from round to irregular. Such objects may be either isolated, clustered, or engulfed in the cytoplasm of a phagocytic cell. Some unstained apoptotic nuclei or bodies may also be expected. Light cytoplasmic staining might co-localize with intense chromatin staining (this might be due to fragmented DNA leaching from the nucleus, or that in phagocytic organelles). If staining of non-apoptotic cell nuclei is observed with the ApopTag® Peroxidase kits (S7100, S7101), the user can attempt to decrease the substrate development time or temperature, or to further dilute the anti-digoxigenin antibody reagent in 0.5% (w:v) BSA. Counterstaining is recommended (see The Complete ApopTag® Manual).
Protein/Isoform Description: One of the terminal and unmistakable steps in apoptosis is endonucleolytic degradation of cellular DNA. Detection of degraded DNA fragments by Terminal Transferase nick-end-labeling (TUNEL) is a commonly-used method for quantitation of apoptosis.
Storage Conditions: Recommended Storage 18°C to 25°C.