规格: 40 Reactions in an 8 Chamber Slide
名称: OxyICC Oxidized Protein Detection Kit 原装正品
Key Applications: Immunofluorescence
Application Notes: Peroxide stressed cell protocol:
Plate cells normal density (typically add ~30k per well of an 8 well chamber slide).
Add fresh media containing H2O2 (500uM treatment for 30 minutes or 50uM treatment overnight)
After appropriate incubation wash cells gently 2X with PBS
Fix cells with ice cold methanol
Continue with the OxyICC protocol.
Components: S7350-1 (Store at 2-8ºC)
Streptavidin-Cy3: (Part No. CS203022) 15µL solution.
2,4-Dinitrophenylhydrazine Solution: (Part No. CS201876) One vial with 1.1mL.
10x PBS Wash Buffer: (Part No. CS201873) One 130mL bottle.
5x Blocking Buffer: (Part No. CS203024) One 10mL bottle.
MILLEX® GP Filter Units (0.22µm): (Part No. SLGP033RB) 3 filters.
S7350-2 (Store at - 20ºC)
Anti-DNP, Biotinylated (mouse monoclonal) Antibody: (Part No. CS203021) 15µL of a 1mg/mL solution.
DAPI: (Part No. CS202687) 10 ?L of 1mg/mL solution.
Storage Conditions: Dual Temperature Storage (See Manual for details) Store for up to 6 months after date of receipt.