上海浩然生物技术有限公司

第7年
4006816680-529
 TRAPeze Telomerase Positive Control Cell Pellet 原装正品
  • TRAPeze Telomerase Positive Control Cell Pellet 原装正品
  • TRAPeze Telomerase Positive Control Cell Pellet 原装正品

    参考价格: ¥1484.00
    品  牌:millipore
    产品型号:S7701 (1 vial)
    所在地区:上海
    上架时间:2018年06月04日
    留言咨询
    4006816680-529
    与企业取得联系时请告知该信息获取自中国教育装备采购网
    我要分享
    详细说明

    TRAPeze Telomerase Positive Control Cell Pellet 原装正品
    产地: millipore
    货号: S7701
    规格: 1 vial
    价格(元):1484
    名称: TRAPeze Telomerase Positive Control Cell Pellet 原装正品
    Application Notes: Instructions for Use
    1. Resuspend the cell pellet in 200 μl of 1X CHAPS Lysis Buffer.
    2. Incubate the suspension on ice for 30 minutes.
    3. Spin the sample in a microcentrifuge at 12,000 X g for 20 minutes at 4°C.
    4. Transfer 160 μl of the supernatant into a fresh tube.
    5. Aliquot and quick-freeze the remaining extract on dry ice, and store at -75°C to -85°C. The extracts for the TRAP assay should be quick-frozen on dry ice after each use. Aliquots should not be freeze-thawed more than 5 times to avoid loss of telomerase activity. Additionally, aliquoting reduces the risk of contamination.
    6. Use 1000 cell equivalents (2 μl of a 1:10 dilution) per TRAP assay, according to the kit manuals.
    Storage Conditions: Must be stored at -75°C to -85°C. Telomerase in specimens stored as a cell pellet is stable at this temperature for several years. Once the cell extract is made (in 1X CHAPS Lysis Buffer), it is stable for 1 year, if not subjected to more than 5 freeze-thaw cycles