上海浩然生物技术有限公司

CpG WIZ VHL Amplification Kit 原装正品

CpG WIZ VHL Amplification Kit 原装正品

参考价格:¥5394.00
品  牌:millipore
产品型号:S7805 (25 assays)
所在地区:上海
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CpG WIZ VHL Amplification Kit 原装正品
产地: millipore
货号: S7805
规格: 25 assays
价格(元):5394
名称: CpG WIZ VHL Amplification Kit 原装正品
Key Applications: PCR
Application Notes: INTRODUCTION
Using This Manual
Please read the entire instruction manual prior to using the CpG WIZ® VHL Amplification Kit. Should additional questions arise, assistance is available from Chemicon Technical Service at techserv@chemicon.com or (800) 437-7500.
Principles of the Technique
MSP, performed using the CpGenome™ DNA Modification Kit and the CpG WIZ® VHL Amplification Kit, permits sensitive detection of altered DNA. Because this is a PCR-based assay, it is extremely sensitive, facilitating the detection of low numbers of methylated alleles and the study of samples containing small amounts of DNA. MSP also allows examination of all CpG sites, not just those within sequences recognized by methylation sensitive restriction enzymes. Increasing the number of such sites which can be assessed allows rapid, fine mapping of methylation patterns throughout CpG regions. In addition, the bisulfite modification is ideally suited for analysis of CpG islands since it converts the majority of cytosines to uracils, making a region of the genome which is CG-rich less difficult to amplify by PCR.
Components: The components of the CpG WIZ® VHL Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.
U Primer Set5 μM each primer (25X) 35 μL (neutral cap) 90516 -15°C to -25°C
M Primer Set5 μM each primer (25X) 35 μL (red cap) 90517 -15°C to -25°C
W Primer Set5 μM each primer (25X) 35 μL (green cap) 90518 -15°C to -25°C
U control DNA0.1 μg/μL 50 μL (white cap) 90393 -15°C to -25°C
M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C
W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C
Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C

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