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原装进口|Inclusion Body Solubilization Reagent

原装进口|Inclusion Body Solubilization Reagent

参考价格:¥2019.00
品  牌:pierce
产品型号:78115 (100ml)
所在地区:华东 上海
上架时间:2017-10-24
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原装进口|Inclusion Body Solubilization Reagent
产地: PIERCE
货号: 78115
规格: 100ml
价格(元):2019
名称: 原装进口|Inclusion Body Solubilization Reagent
The Inclusion Body Solubilization Reagent is a ready-to-use aqueous solution of denaturant that helps to dissolve aggregated proteins (inclusion bodies) that have been separated from the soluble fraction of bacterial cell lysates. The reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents such as Thermo Scientific B-PER Bacterial Protein Extraction Reagent. Bacteria are widely used for recombinant protein expression. However, 70%-80% of proteins produced by recombinant techniques in E. coli form inclusion bodies (i.e., protein aggregates). Once these aggregates are formed, it is very difficult to solubilize them. A proprietary denaturant contained in this reagent provides the most effective means for solubilizing aggregated proteins. Additional components, such as a reducing agent and a chelating agent, may be added to the reagent, depending on the particular application.
Highlights:
Proprietary denaturant can be easily removed by dialysis so that SDS-PAGE can be performed
Can accommodate additional components such as reducing agents or chelating agents
Achieves the essential first step for subsequent insoluble protein refolding procedures
One easy- and ready-to-use solution; no preparation needed
Compatible with Thermo Scientific Pierce Coomassie Plus Protein Assay Kit (Part No. 23236) to determine degree of expression

产地
货号
规格
名称
价格(元)
PIERCE
77110
5 x 20mg
Imject BSA (in PBS)
1885
PIERCE
77115
10mg
Imject Maleimide-Activated BSA
3709
PIERCE
77116
2mg
Imject Maleimide-Activated BSA
950
PIERCE
77125
10mg
Imject Maleimide-Activated Ovalbumin
3709
PIERCE
77126
2mg
Imject Maleimide-Activated Ovalbumin
950
PIERCE
77150
10mg
Imject cBSA (in PBS)
3048
PIERCE
77159
5g
Imject Purification Buffer Salts
330
PIERCE
77164
30ml
Imject Maleimide Conjugation Buffer
543
PIERCE
77171
2mg
Imject BSA (in MES Buffer)
475
PIERCE
77606
2mg
Imject Maleimide-Activated mcKLH
1029
PIERCE
77610
10 x 10mg
Imject Maleimide-Activated mcKLH
34404
PIERCE
77649
20mg
Imject mcKLH Subunits, High Purity Research Grade
3515
PIERCE
77660
500mg
Imject Blue Carrier Protein
2771
PIERCE
77661
2mg
Imject Maleimide-Activated Blue Carrier Protein
659
PIERCE
77662
10 x 10mg
Imject Maleimide-Activated Blue Carrier Protein
6975
PIERCE
77663
10mg
Imject Maleimide PEGylated mcKLH
3960
PIERCE
77664
10mg
Imject Maleimide-Activated Blue Carrier Protein
2969
PIERCE
77665
5 x 2mg kit
Imject Maleimide-Activated Blue Carrier Protein Spin Kit
3695
PIERCE
77666
5 x 2mg kit
Imject Maleimide-Activated mcKLH Spin Kit
8145
PIERCE
77667
5 x 2mg kit
Imject Maleimide-Activated BSA Spin Kit
4751
PIERCE
77670
5 x 2mg kit
Imject EDC Blue Carrier Protein Spin Kit
3695
PIERCE
77671
5 x 2mg kit
Imject EDC mcKLH Spin Kit
4751
PIERCE
77672
5 x 2mg kit
Imject EDC BSA Spin Kit
4751
PIERCE
77701
1 x 2ml
Immobilized Reductant Column
2429
PIERCE
77712
5ml
Immobilized TCEP Disulfide Reducing Gel
1768
PIERCE
78110
45ml
Wash Buffer 1 for B-PER 6xHis Kit
647
PIERCE
78115
100ml
Inclusion Body Solubilization Reagent
2019
PIERCE
78120
60ml
Wash Buffer 2 for B-PER 6xHis Kit
633
PIERCE
78130
45ml
Elution Buffer for 6xHis Kit
633
PIERCE
78248
500ml
B-PER Bacterial Protein Extraction Reagent
3353
PIERCE
78256
85ml
Wash Buffer 2 for GST Purification Kits
518
PIERCE
78257
60ml
Wash Buffer 1 for GST Purification Kits
518
PIERCE
78259
5 x 184mg
Glutathione
729
PIERCE
78260
250ml
B-PER II Bacterial Protein Extraction Reagent (2X)
3418
PIERCE
78266
500ml
B-PER Reagent (in Phosphate Buffer)
3418
PIERCE
78320
8ml
Nickel Chelated Agarose for 6xHis Kit
2283