上海浩然生物技术有限公司

 原装进口|Inclusion Body Solubilization Reagent
  • 原装进口|Inclusion Body Solubilization Reagent
  • 原装进口|Inclusion Body Solubilization Reagent

    参考价格: ¥2019.00
    品  牌:pierce
    产品型号:78115 (100ml)
    所在地区:上海
    上架时间:2018年11月01日
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    原装进口|Inclusion Body Solubilization Reagent
    产地: PIERCE
    货号: 78115
    规格: 100ml
    价格(元):2019
    名称: 原装进口|Inclusion Body Solubilization Reagent
    The Inclusion Body Solubilization Reagent is a ready-to-use aqueous solution of denaturant that helps to dissolve aggregated proteins (inclusion bodies) that have been separated from the soluble fraction of bacterial cell lysates. The reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents such as Thermo Scientific B-PER Bacterial Protein Extraction Reagent. Bacteria are widely used for recombinant protein expression. However, 70%-80% of proteins produced by recombinant techniques in E. coli form inclusion bodies (i.e., protein aggregates). Once these aggregates are formed, it is very difficult to solubilize them. A proprietary denaturant contained in this reagent provides the most effective means for solubilizing aggregated proteins. Additional components, such as a reducing agent and a chelating agent, may be added to the reagent, depending on the particular application.
    Highlights:
    Proprietary denaturant can be easily removed by dialysis so that SDS-PAGE can be performed
    Can accommodate additional components such as reducing agents or chelating agents
    Achieves the essential first step for subsequent insoluble protein refolding procedures
    One easy- and ready-to-use solution; no preparation needed
    Compatible with Thermo Scientific Pierce Coomassie Plus Protein Assay Kit (Part No. 23236) to determine degree of expression

    产地
    货号
    规格
    名称
    价格(元)
    PIERCE
    77110
    5 x 20mg
    Imject BSA (in PBS)
    1885
    PIERCE
    77115
    10mg
    Imject Maleimide-Activated BSA
    3709
    PIERCE
    77116
    2mg
    Imject Maleimide-Activated BSA
    950
    PIERCE
    77125
    10mg
    Imject Maleimide-Activated Ovalbumin
    3709
    PIERCE
    77126
    2mg
    Imject Maleimide-Activated Ovalbumin
    950
    PIERCE
    77150
    10mg
    Imject cBSA (in PBS)
    3048
    PIERCE
    77159
    5g
    Imject Purification Buffer Salts
    330
    PIERCE
    77164
    30ml
    Imject Maleimide Conjugation Buffer
    543
    PIERCE
    77171
    2mg
    Imject BSA (in MES Buffer)
    475
    PIERCE
    77606
    2mg
    Imject Maleimide-Activated mcKLH
    1029
    PIERCE
    77610
    10 x 10mg
    Imject Maleimide-Activated mcKLH
    34404
    PIERCE
    77649
    20mg
    Imject mcKLH Subunits, High Purity Research Grade
    3515
    PIERCE
    77660
    500mg
    Imject Blue Carrier Protein
    2771
    PIERCE
    77661
    2mg
    Imject Maleimide-Activated Blue Carrier Protein
    659
    PIERCE
    77662
    10 x 10mg
    Imject Maleimide-Activated Blue Carrier Protein
    6975
    PIERCE
    77663
    10mg
    Imject Maleimide PEGylated mcKLH
    3960
    PIERCE
    77664
    10mg
    Imject Maleimide-Activated Blue Carrier Protein
    2969
    PIERCE
    77665
    5 x 2mg kit
    Imject Maleimide-Activated Blue Carrier Protein Spin Kit
    3695
    PIERCE
    77666
    5 x 2mg kit
    Imject Maleimide-Activated mcKLH Spin Kit
    8145
    PIERCE
    77667
    5 x 2mg kit
    Imject Maleimide-Activated BSA Spin Kit
    4751
    PIERCE
    77670
    5 x 2mg kit
    Imject EDC Blue Carrier Protein Spin Kit
    3695
    PIERCE
    77671
    5 x 2mg kit
    Imject EDC mcKLH Spin Kit
    4751
    PIERCE
    77672
    5 x 2mg kit
    Imject EDC BSA Spin Kit
    4751
    PIERCE
    77701
    1 x 2ml
    Immobilized Reductant Column
    2429
    PIERCE
    77712
    5ml
    Immobilized TCEP Disulfide Reducing Gel
    1768
    PIERCE
    78110
    45ml
    Wash Buffer 1 for B-PER 6xHis Kit
    647
    PIERCE
    78115
    100ml
    Inclusion Body Solubilization Reagent
    2019
    PIERCE
    78120
    60ml
    Wash Buffer 2 for B-PER 6xHis Kit
    633
    PIERCE
    78130
    45ml
    Elution Buffer for 6xHis Kit
    633
    PIERCE
    78248
    500ml
    B-PER Bacterial Protein Extraction Reagent
    3353
    PIERCE
    78256
    85ml
    Wash Buffer 2 for GST Purification Kits
    518
    PIERCE
    78257
    60ml
    Wash Buffer 1 for GST Purification Kits
    518
    PIERCE
    78259
    5 x 184mg
    Glutathione
    729
    PIERCE
    78260
    250ml
    B-PER II Bacterial Protein Extraction Reagent (2X)
    3418
    PIERCE
    78266
    500ml
    B-PER Reagent (in Phosphate Buffer)
    3418
    PIERCE
    78320
    8ml
    Nickel Chelated Agarose for 6xHis Kit
    2283