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 EZ-Link Biotin-PEG4-Hydrazide 现货
  • EZ-Link Biotin-PEG4-Hydrazide 现货
  • EZ-Link Biotin-PEG4-Hydrazide 现货

    参考价格: ¥3996.00
    品  牌:Pierce
    产品型号:21360
    所在地区:上海
    上架时间:2018年11月01日
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    EZ-Link Biotin-PEG4-Hydrazide 现货
    产品: Pierce
    货号: 21360
    规格: 50 MG
    名称: EZ-Link Biotin-PEG4-Hydrazide
    Thermo Scientific EZ-Link Hydrazide-PEG4-Biotin (also called Biotin-PEG4-Hydrazide) is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol (PEG) spacer arm for increased hydrophilicity.
    Biotin-PEG4-Hydrazide enables biotinylation of glycoproteins and other molecules containing aldehydes or oxidizable sugar groups. The hydrazide group reacts spontaneously with aldehyde or ketone groups to form a stable hydrazone bond. Aldehyde groups can be created by gentle periodate oxidation of cis-diols in sialic acid and other sugars in carbohydrates. The hydrophilic, 4-unit polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG segment also adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules.
    References:
    Edwards, S.W., et al. (1999). J. Biol. Chem. 274, 16331-16336.
    Hermanson, G.T. (1996). Bioconjugate Techniques, Academic Press (Product # 20002).
    Scott, M.G., et al. (2000). J. Immunol. 164(2), 549-553.
    Highlights:
    Carbohydrate-reactive – forms stable hydrazone bond with periodate-oxidized sialic acid and other sugar groups in glycoproteins
    Good choice for labeling polyclonal antibodies in the carbohydrate-rich Fc region, thereby avoiding steric hindrance of the antigen-binding sites
    Use to biotinylate cell surface glycoproteins
    PEGylation increases solubility and helps prevent aggregation of labeled molecules
    PEG spacer arm reduces steric hindrance when binding to avidin molecules
    Reactive group: hydrazide
    Reactive toward: aldehyde and ketone groups (oxidized cis-diols in glycoproteins)