规格: 50 MG
名称: EZ-Link Biotin-PEG4-Hydrazide
Thermo Scientific EZ-Link Hydrazide-PEG4-Biotin (also called Biotin-PEG4-Hydrazide) is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol (PEG) spacer arm for increased hydrophilicity.
Biotin-PEG4-Hydrazide enables biotinylation of glycoproteins and other molecules containing aldehydes or oxidizable sugar groups. The hydrazide group reacts spontaneously with aldehyde or ketone groups to form a stable hydrazone bond. Aldehyde groups can be created by gentle periodate oxidation of cis-diols in sialic acid and other sugars in carbohydrates. The hydrophilic, 4-unit polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG segment also adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules.
Edwards, S.W., et al. (1999). J. Biol. Chem. 274, 16331-16336.
Hermanson, G.T. (1996). Bioconjugate Techniques, Academic Press (Product # 20002).
Scott, M.G., et al. (2000). J. Immunol. 164(2), 549-553.
Carbohydrate-reactive – forms stable hydrazone bond with periodate-oxidized sialic acid and other sugar groups in glycoproteins
Good choice for labeling polyclonal antibodies in the carbohydrate-rich Fc region, thereby avoiding steric hindrance of the antigen-binding sites
Use to biotinylate cell surface glycoproteins
PEGylation increases solubility and helps prevent aggregation of labeled molecules
PEG spacer arm reduces steric hindrance when binding to avidin molecules
Reactive group: hydrazide
Reactive toward: aldehyde and ketone groups (oxidized cis-diols in glycoproteins)