名称: EZ-Link Sulfo-NHS Biotinylation Kit
Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
EZ-Link Sulfo-NHS-Biotin Reagents enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.
Label almost any antibody with biotin to facilitate immobilization, purification or detection
Short spacer arm (13.5 angstroms) adds minimal mass to tagged molecules (226.3 daltons)
Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
Sulfonate group attached to the biotinylation reagents increases reagent water solubility compared to ordinary NHS esters
Sulfo-NHS-Biotin is water soluble, so biotin labeling can be done under physiologic conditions
High purity, crystalline biotin labeling reagents can be used to create high-quality biotin-labeled derivatives
We manufacture biotinylation agents to the highest specifications to produce the most specific biotin labeled proteins, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of a biotin labeling reagent is tested to meet our purity and solubility specifications.
N -Hydroxysulfosuccinimide (Sulfo-NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amines (-NH2) in pH 7-9 buffers to form stable amide bonds. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for labeling with NHS-activated biotin reagents. Several different NHS esters of biotin are available, with varying properties and spacer arm lengths. The sulfo-NHS ester reagents are water soluble, enabling reactions to be performed in the absence of organic solvents such as DMSO or DMF.