上海浩然生物技术有限公司

 EZ-Link Sulfo-NHS Biotinylation Kit 现货
  • EZ-Link Sulfo-NHS Biotinylation Kit 现货
  • EZ-Link Sulfo-NHS Biotinylation Kit 现货

    参考价格: ¥3208.00
    品  牌:Pierce
    产品型号:21425
    所在地区:上海
    上架时间:2018年11月01日
    留言咨询
    与企业取得联系时请告知该信息获取自中国教育装备采购网
    我要分享
    详细说明

    EZ-Link Sulfo-NHS Biotinylation Kit 现货
    产品: Pierce
    货号: 21425
    规格: KIT
    名称: EZ-Link Sulfo-NHS Biotinylation Kit 
    Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
    EZ-Link Sulfo-NHS-Biotin Reagents enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.
    Highlights:
    Label almost any antibody with biotin to facilitate immobilization, purification or detection
    Short spacer arm (13.5 angstroms) adds minimal mass to tagged molecules (226.3 daltons)
    Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
    Sulfonate group attached to the biotinylation reagents increases reagent water solubility compared to ordinary NHS esters
    Sulfo-NHS-Biotin is water soluble, so biotin labeling can be done under physiologic conditions
    High purity, crystalline biotin labeling reagents can be used to create high-quality biotin-labeled derivatives
    Product Details:
    We manufacture biotinylation agents to the highest specifications to produce the most specific biotin labeled proteins, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of a biotin labeling reagent is tested to meet our purity and solubility specifications. 
    N -Hydroxysulfosuccinimide (Sulfo-NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amines (-NH2) in pH 7-9 buffers to form stable amide bonds. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for labeling with NHS-activated biotin reagents. Several different NHS esters of biotin are available, with varying properties and spacer arm lengths. The sulfo-NHS ester reagents are water soluble, enabling reactions to be performed in the absence of organic solvents such as DMSO or DMF.