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 Restore Plus WB Stripping Buffer 现货
  • Restore Plus WB Stripping Buffer 现货
  • Restore Plus WB Stripping Buffer 现货

    参考价格: ¥1341.00
    品  牌:Pierce
    产品型号:46430
    所在地区:上海
    上架时间:2018年11月01日
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    Restore Plus WB Stripping Buffer
    Restore Plus WB Stripping Buffer 现货
    产品: Pierce
    货号: 46430
    规格: 500 ML
    名称: Restore Plus WB Stripping Buffer
    Thermo Scientific Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
    Restore PLUS Buffer is an alternative formulation of the original Thermo Scientific Restore Western Blot Stripping Buffer. Restore PLUS Stripping Buffer was designed for use with antibodies that are difficult to remove from Western blots and require longer incubation times or incubation temperatures greater than 22°C with gentler formulations. High-affinity antibodies can be quickly and effectively stripped from Western blots at room temperature without removing transferred proteins, thereby allowing multiple reprobes of the target.
    Protocol Summary:
    Wash blot to remove chemiluminescent substrate
    Incubate blot in Restore Western Blot Stripping Buffer for 5 to 15 minutes at room temperature
    (or incubate at 37°C for high affinity antibodies)
    Remove blot and wash in Wash Buffer
    Block membrane
    Test for sufficient removal of antibodies
    Perform next immunoblot experiment
    Highlights:
    Ready and easy to use – no dilution necessary; no offensive odors; store at room temperature
    Compatible – use on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate and chemiluminescent substrate
    Cost effective – save valuable time and samples; strip blots effectively the first time
    Robust yet gentle – transferred proteins remain viable; strip the same blot up to five times
    Flexible – strip and reprobe to optimize antibody concentrations or to detect a new antigen with different antibodies
    Efficient – enhanced yield and sample purity