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 Mitochondria Isolation Kit 原装现货
  • Mitochondria Isolation Kit 原装现货
  • Mitochondria Isolation Kit 原装现货

    参考价格: ¥2406.00
    品  牌:Pierce
    产品型号:89874
    所在地区:上海
    上架时间:2018年11月01日
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    Mitochondria Isolation Kit 原装现货
    产品: Pierce
    货号: 89874
    规格: KIT
    名称: Mitochondria Isolation Kit
    The Thermo Scientific Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.
    Isolation of mitochondria is typically a laborious process requiring single-sample processing with Dounce homogenization. The Mitochondria Isolation Kit uses a non-mechanical, reagent-based method that allows multiple (six) cultured cell samples to be processed concurrently in about 40 minutes. Cultured mammalian cell pellets are gently lysed using a proprietary formulation that results in maximum yield of mitochondria with minimal damage to integrity. The product instructions describe guidelines for optimizing purity vs. yield parameters. The kit also offers an optimized Dounce homogenization procedure, which results in two-fold greater mitochondria recovery compared to the reagent-based method. Both methods use differential centrifugation to separate the mitochondrial and cytosolic fractions with a benchtop microcentrifuge and are completed in approximately 40 minutes (post-cell harvest). Once isolated, the mitochondria can be used in downstream applications such as apoptosis, signal transduction and metabolic studies, as well as to facilitate mitochondrial proteomics efforts.
    Highlights:
    Fast – isolate intact mitochondria from 20 million cells in approximately 40 minutes (post-cell harvest)
    Flexible – process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method
    Optimizable – guidelines provided for optimizing purity vs. yield with the reagent-based method
    Benchtop-easy – isolation performed in a microcentrifuge tube
    Compatible – isolated mitochondria may be lysed with detergent or processed for almost any downstream application, including 2D electrophoresis