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 LIF MS -10UG 原装现货
  • LIF MS -10UG 原装现货
  • LIF MS -10UG 原装现货

    参考价格: ¥3020.00
    品  牌:Millipore
    产品型号:LIF2010
    所在地区:上海
    上架时间:2018年11月01日
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    LIF MS -10UG 原装现货
    产品: Millipore
    货号: LIF2010
    名称: LIF, MS -10UG
    Product Overview: Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is a 22 kDa protein containing 203 amino acid residues.
    Application Notes: RECOMMENDED QC PROTOCOL
    M1 Bioassay
    1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
    2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
    3. Add 100 μL of 5% FCS in isotonic saline to two control slides.
    4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
    5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
    Visit www.esgro-lif.com for additional information
    Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.