名称: 正品|Ciliary Neurotrophic Factor Recombinant Human (CNTF)
Recombinant Human CNTF is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22,706 Daltons.
Source E. coli
Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Thr-Glu.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin 0.1ng/ug (IEU/ug)
Protein Content Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of human CNTF as a Reference Standard.
Biological Activity Recombinant Human Ciliary Neurotrophic Factor is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less then 2 ng/ml, corresponding to a Specific Activity of 5 x 10 IU/mg.
Reconstitution Recommended to reconstitute the lyophilized CNTF in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and Stability Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source E. coli
Purity 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.