上海浩然生物技术有限公司

 AFA I (Rsa I)原装正品
  • AFA I (Rsa I)原装正品
  • AFA I (Rsa I)原装正品

    参考价格: ¥572.00
    品  牌:invitrogen
    产品型号:15240401
    所在地区:上海
    上架时间:2019年01月11日
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    AFA I (Rsa I)原装正品
    产品: invitrogen
    货号: 15240401
    规格: 1000 UN
    名称: Afa I (Rsa I)
    Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
    Concentration 10 U/µL
    Contents Afa I
    10X Buffer T (1 mL)
    0.1% BSA (1 mL)
    10X Loading Buffer (1 mL) 
    Reaction Temperature 37°C
    Reaction Mixture  Afa I 1 µL
    10X Buffer T 2 µL
    0.1% BSA 2 µL
    Substrate DNA ≤ 1µg
    Sterile water to 20 µL
    Heat inactivation Enzyme is inactivated by heating at 65°C for 20 minutes.
    Star Activity  
    Effect of DNA Methylation Enzyme activity is often affected by 
    CG methylase.
    Unit Definition One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
    10X Buffer T (BSA free) 330 mM Tris-AC, pH 7.9
    100 mM Mg-Ac
    5 mM Dithiothreitol
    660 mM K-Ac
    Source Acidophilium facilis
    For Research Use Only. Not for any animal or human therapeutic or diagnostic use.