名称: 正品|Insulin-Like Growth Factor I, Recombinant, Mouse (IGF1)
Recombinant murine IGF-1 is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kD.
AA Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically.
Biological Activity IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is 1.0ng/ml, corresponding to a specific activity of 1x10e5 U/mg
Protein Content Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IGF-I as a Reference Standard.
Storage and Stability Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.