产品名称：ANTI-AQUAPORIN 1 (243-261) RABBIT PAB
Application Notes: Immunoblotting (1-2 μg/ml, chemiluminescence)
Note: The epitope is unique for AQP1 and is not present in other known related proteins. It is identical in human, rodent, and cattle, and only slightly different in frog. Recognition of AQP1 can be fully inhibited with the soluble AQP1243-261 blocking peptide. For use as a negative control, preincubate 1 μg blocking peptide with 1 μg antibody for 1 h at room temperature. This antibody is also reported to work with frozen sections. Antibody should be titrated for optimal results in individual systems.
Presentation: Lyophilized; 50 μl antibody lyophilized from PBS, pH 7.4 and 40 μg blocking peptide lyophilized from PBS, containing ≤0.1% sodium azide (antibody only).
Storage Conditions: Dissolve the antibody in 50 μl deionized water to make a 1 mg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of stock solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Dissolve the peptide in 100 μl deionized water and store in aliquots at -20°C.