Background Information: A cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.
Molecular Weight: 315.4
Presentation: Pink solid. Packaged under inert gas.
Storage Conditions: Shipped at Ambient Temperature. Store at -20°C.
Properties: Solubility: DMF or DMSO (20 mg/ml).
RTCS No.: SF7935000
Empirical Formula: C21H21N3
Purity: ≥95% by HPLC