上海浩洋生物科技有限公司

 ENDOPEPTIDASE LYS-C, A. LYTICUS
  • ENDOPEPTIDASE LYS-C, A. LYTICUS
  • ENDOPEPTIDASE LYS-C, A. LYTICUS

    参考价格: ¥3036.00
    品  牌:merck
    产品型号:324796-2U (2U)
    所在地区:上海
    上架时间:2013年04月25日
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    详细说明

    ENDOPEPTIDASE LYS-C, A. LYTICUS
    产品名称:ENDOPEPTIDASE LYS-C, A. LYTICUS
    产品货号:324796-2U
    产品价格:¥3036
    产品规格:2U
    Application Notes: Amidase Activity:
    Materials Required:
    •Reagent A: 200 mM AMP, pH 9.5 (dissolve 4.2 g 2-amino-2-methyl-1,3-propanediol in 150 ml H2O; adjust the pH to 9.5 with 1 M HCl; add H2O to a final volume of 200 ml)
    •Reagent B: 2.5 mM Bz-Lys-pNA (dissolve 22.6 mg Nα-benzoyl-DL-lysine-p-nitroanilide in 900 ml H2O)
    •Reagent C: enzyme diluent (dissolve 240 µg 2-amino-2-hydroxymethyl-1,3-propanediol in 900 ml H2O; adjust the pH to 8.0 with 0.1 M HCl; add H2O to a final volume of 1 L
    •Reagent D: enzyme solution (dissolve 1 vial enzyme in 1 ml H2O; dilute 100 µl to a final volume of 25 ml with Reagent C)
    •45% Acetic Acid (stop solution)
    Protocol
    1. Add 2.6 ml Reagent A to 300 µl Reagent B and pre-incubate at 30°C to equilibrate the temperature.
    2. Add 100 µl Reagent D and incubate for 25 min at 30°C. Include 100 µl Reagent C alone as a control.
    3. Add 1 ml 45% acetic acid to stop the reaction.
    4. Measure the absorbance at 405 nm.
    5. Calculate the activity:
    Amidase Activity (AU/vial) = [(Atest-Acontrol)/25] x [1/9.62] x [4/0.1] x 250
    Molecular Weight: 30000
    Presentation: Lyophilized.
    Properties: Solubility: H2O or Tris-HCl buffer, pH 4-11. Reconstitute 1 vial with 1 ml solvent; if the enzyme is not completely solubilized, add 1-3 ml additional solvent until completely dissolved.
    Specific Activity: ≥2 AU/mg protein
    Storage Conditions: Shipped on Blue Ice. Store at -20°C. Avoid freeze/thaw.